{"id":302362,"date":"2024-10-19T20:39:23","date_gmt":"2024-10-19T20:39:23","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-iso-ts-190062016\/"},"modified":"2024-10-25T18:10:14","modified_gmt":"2024-10-25T18:10:14","slug":"bsi-pd-iso-ts-190062016","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-iso-ts-190062016\/","title":{"rendered":"BSI PD ISO\/TS 19006:2016"},"content":{"rendered":"

This document describes how to test and evaluate results obtained from in vitro<\/i> ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2<\/sub>DCFDA assay.<\/p>\n

The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
11<\/td>\n4 Symbols and abbreviated terms
5 Materials <\/td>\n<\/tr>\n
13<\/td>\n6 Technical equipment
7 Nanoparticle sample preparation <\/td>\n<\/tr>\n
14<\/td>\n8 Preparations
8.1 General <\/td>\n<\/tr>\n
15<\/td>\n8.2 Flow cytometry calibration
8.3 Experimental culture medium
8.4 Reagent preparation
8.5 Preparation of cell stock culture
8.6 Preparing culture for experiments <\/td>\n<\/tr>\n
16<\/td>\n8.7 Verification of healthy cell growth <\/td>\n<\/tr>\n
17<\/td>\n8.8 Evaluation of nanoparticle interference
8.9 Control preparation
8.9.1 General
8.9.2 Control description <\/td>\n<\/tr>\n
18<\/td>\n8.9.3 Sin-1 stock solution preparation (1\u00a0mM)
9 Evaluation of nanoparticle impact on ROS generation in cells
9.1 Prepare cells in the 24 well plates
9.2 Dose the cells with nanoparticles and controls <\/td>\n<\/tr>\n
19<\/td>\n9.3 Expose the cells to CM-H2DCF-DA Assay <\/td>\n<\/tr>\n
20<\/td>\n9.4 Incubate the cells with CM-H2DCF-DA
9.5 Flow cytometry analysis
10 Data analysis and results <\/td>\n<\/tr>\n
22<\/td>\nAnnex\u00a0A (informative) Alternate cell lines <\/td>\n<\/tr>\n
23<\/td>\nAnnex\u00a0B (informative) Alternate fluorescence characterization techniques <\/td>\n<\/tr>\n
24<\/td>\nAnnex\u00a0C (informative) Suspension preparation and characterization <\/td>\n<\/tr>\n
25<\/td>\nAnnex\u00a0D (informative) Example experimental data from RAW 264.7 <\/td>\n<\/tr>\n
28<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Nanotechnologies. 5-(and 6)-Chloromethyl-2\u2019,7\u2019 Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2016<\/td>\n32<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":302367,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-302362","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/302362","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/302367"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=302362"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=302362"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=302362"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}