{"id":379614,"date":"2024-10-20T03:03:14","date_gmt":"2024-10-20T03:03:14","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-237342021\/"},"modified":"2024-10-26T05:29:22","modified_gmt":"2024-10-26T05:29:22","slug":"bs-iso-237342021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-237342021\/","title":{"rendered":"BS ISO 23734:2021"},"content":{"rendered":"

This document specifies a bioassay for the determination of the presence of unknown toxic contaminants in test seawater (see Figure A.1<\/span>). It is based on the inhibition of photosynthetic activity of the marine cyanobacterium Cyanobium<\/i> sp. (NIES-981) by such toxic contaminants. The inhibition is determined based on delayed fluorescence (DF) intensity.<\/p>\n

The method is rapid and requires less laboratory space than standard bioassays. Hence, it can be used on-board to generate basic data for seawater quality management at deep-sea mining sites where time and space are extremely limited.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4 Principle <\/td>\n<\/tr>\n
11<\/td>\n5 Materials
5.1 Test alga
5.2 Reagents
5.2.1 Water
5.2.2 Growth medium
5.2.3 Nutrient, metal and tris stock solutions <\/td>\n<\/tr>\n
12<\/td>\n6 Apparatus
6.1 General
6.2 High sensitivity luminometer
6.3 Incubator and tube shaker
6.4 Apparatus for determining algal cell density
6.5 Culture tubes
6.6 Clean bench
7 Preparations at a land-based laboratory
7.1 Preparation of the growth medium <\/td>\n<\/tr>\n
13<\/td>\n7.2 Preparation of the algal stock culture
8 Test procedure on-board
8.1 Preparation of the algal inoculum culture
8.2 Choice of the test concentrations
8.3 Preparation of the test medium <\/td>\n<\/tr>\n
14<\/td>\n8.4 Inoculation and incubation
9 DF measurement
10 Interpretation of data
10.1 Plotting the DF decay curve
10.2 Calculation of per cent inhibition <\/td>\n<\/tr>\n
15<\/td>\n11 Interpretation of the results
12 Test report <\/td>\n<\/tr>\n
17<\/td>\nAnnex A (informative) Schematic overview and procedures of the on-board bioassay <\/td>\n<\/tr>\n
20<\/td>\nAnnex B (informative) Preparation of the test medium in seawater <\/td>\n<\/tr>\n
21<\/td>\nAnnex C (informative) Practical procedures for the on-board bioassay and schematic diagram of the high sensitivity luminometer <\/td>\n<\/tr>\n
22<\/td>\nAnnex D (informative) Cryopreservation procedure <\/td>\n<\/tr>\n
23<\/td>\nAnnex E (informative) Reference data for checking the appropriateness of the test procedures <\/td>\n<\/tr>\n
24<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Marine technology. Marine environment impact assessment (MEIA). On-board bioassay to monitor seawater quality using delayed fluorescence of microalga<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2021<\/td>\n26<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":379622,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[784,2641],"product_tag":[],"class_list":{"0":"post-379614","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-47-020-01","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/379614","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/379622"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=379614"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=379614"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=379614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}