This document gives general guidelines for enumeration of yeast and mould present in cosmetics by counting the colonies on selective agar medium after aerobic incubation.<\/p>\n
In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621 ) include those with low water activity or extreme pH values, hydro-alcoholic products, etc.<\/p>\n
Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other methods (e.g. automated) can be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.<\/p>\n
Yeast enumerated can be identified using suitable identification tests, for example, tests described in the standards listed in the Bibliography. Mould enumerated can be identified by other appropriate methods, if necessary.<\/p>\n
| PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
|---|---|---|---|---|---|---|---|
| 2<\/td>\n | National foreword <\/td>\n<\/tr>\n | ||||||
| 6<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
| 9<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions <\/td>\n<\/tr>\n | ||||||
| 10<\/td>\n | 4 Principles 4.1 General 4.2 Plate count 4.3 Membrane filtration <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | 5 Diluents, neutralizers and culture media 5.1 General 5.2 Neutralizing diluents and diluents <\/td>\n<\/tr>\n | ||||||
| 12<\/td>\n | 5.3 Diluent for yeast suspension (tryptone sodium chloride solution) 5.4 Culture media <\/td>\n<\/tr>\n | ||||||
| 13<\/td>\n | 6 Apparatus and glassware 7 Strain of microorganisms <\/td>\n<\/tr>\n | ||||||
| 14<\/td>\n | 8 Handling of cosmetic products and laboratory samples 9 Procedure 9.1 General recommendation 9.2 Preparation of the initial suspension 9.2.1 General 9.2.2 Water-miscible products 9.2.3 Water-immiscible products 9.3 Counting methods 9.3.1 Dilutions for counting methods <\/td>\n<\/tr>\n | ||||||
| 15<\/td>\n | 9.3.2 Plate-count methods 10 Counting of colonies (plate counts and membrane filtration methods) <\/td>\n<\/tr>\n | ||||||
| 16<\/td>\n | 11 Expression of results 11.1 Method of calculation for plate count <\/td>\n<\/tr>\n | ||||||
| 17<\/td>\n | 11.2 Interpretation <\/td>\n<\/tr>\n | ||||||
| 18<\/td>\n | 12 Neutralization of the antifungicidal properties of the product 12.1 General <\/td>\n<\/tr>\n | ||||||
| 19<\/td>\n | 12.2 Preparation of inoculum 12.3 Suitability of counting methods 12.3.1 Principle 12.3.2 Suitability test of the pour-plate method 12.3.3 Suitability of the surface spread method 12.3.4 Suitability of the membrane filtration method <\/td>\n<\/tr>\n | ||||||
| 20<\/td>\n | 13 Test report <\/td>\n<\/tr>\n | ||||||
| 21<\/td>\n | Annex\u00a0A (informative) Other neutralizing diluents <\/td>\n<\/tr>\n | ||||||
| 23<\/td>\n | Annex\u00a0B (informative) Other diluents <\/td>\n<\/tr>\n | ||||||
| 24<\/td>\n | Annex\u00a0C (informative) Other culture media <\/td>\n<\/tr>\n | ||||||
| 26<\/td>\n | Annex\u00a0D (informative) Neutralizers of antifungicidal activity of preservatives and rinsing liquids <\/td>\n<\/tr>\n | ||||||
| 27<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Cosmetics. Microbiology. Enumeration of yeast and mould<\/b><\/p>\n |