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BS EN ISO 6888-1:2021

BS EN ISO 6888-1:2021

$142.49

Microbiology of the food chain. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) – Method using Baird-Parker agar medium

Published By Publication Date Number of Pages
BSI 2021 30
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This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (Baird-Parker medium)[ 10 ] after aerobic incubation at 34 °C to 38 °C and coagulase confirmation.

This document is applicable to:

  • products intended for human consumption;

  • products intended for animal feeding;

  • environmental samples in the area of food and feed production, handling, and

  • samples from the primary production stage.

This horizontal method was originally developed for the examination of all samples belonging to the food chain.

Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.

Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on Staphylococcus spp (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:

  • staphylococci forming atypical colonies on a Baird-Parker agar medium;

  • background flora that can obscure the colonies being sought.

Nevertheless, both this document and ISO 6888-2 are given equivalent status.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
8 Introduction
9 1 Scope
2 Normative references
10 3 Terms and definitions
4 Principle
4.1 General
4.2 Incubation
4.3 Enumeration and confirmation
11 5 Culture media and reagents
6 Equipment and consumables
6.1 Apparatus for dry sterilization (oven) and wet sterilization (autoclave).
12 7 Sampling
8 Preparation of the test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
13 9.3 Counting of colonies
9.3.1 General description of colonies growing on BPA medium
9.3.2 Colony counting procedure
14 9.4 Confirmation
9.4.1 General
9.4.2 Tube test
15 9.4.3 Plate test using RPFA medium
10 Expression of results
16 11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Repeatability limit
11.3 Reproducibility limit
17 12 Test report
13 Quality assurance
18 Annex A (normative) Flow diagram of the procedure
19 Annex B (normative) Culture media and reagents
26 Annex C (informative) Results of the interlaboratory study
28 Bibliography

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BS EN ISO 6888-1:2021
$142.49