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BS EN ISO 4833-1:2013

BS EN ISO 4833-1:2013

$102.76

Microbiology of the food chain. Horizontal method for the enumeration of microorganisms – Colony count at 30 degrees C by the pour plate technique

Published By Publication Date Number of Pages
BSI 2013 18
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This part of ISO 4833 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 Ā°C. The method is applicable to:

  1. products intended for human consumption and for animal feed;

  2. environmental samples in the area of food and feed production and handling.

This part of ISO 4833 is applicable to:

  1. products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples);

  2. products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp.

The applicability of this part of ISO 4833 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively.

For some matrices, the method specified in this part of ISO 4833 can give different results to those obtained using the method specified in ISO 4833-2.

PDF Catalog

PDF Pages PDF Title
6 Foreword
7 Section sec_1
Section sec_2
Section sec_3
1 Scope
2 Normative references
3 Terms and definitions
8 Section sec_3.1
Section sec_4
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
4 Principle
5 Culture media and diluents
5.1 General
5.2 Diluents
5.3 Agar medium: plate count agar (PCA)
9 Section sec_5.3.3
Section sec_5.3.3.1
Section sec_5.3.3.2
Section sec_5.4
Section sec_5.4.1
Section sec_5.4.2
5.4 Overlay medium (if necessary; see 9.2.7)
10 Section sec_6
Section sec_6.1
Section sec_6.2
Section sec_6.3
Section sec_6.4
Section sec_6.5
Section sec_6.6
Section sec_6.7
Section sec_6.8
Section sec_7
Section sec_8
Section sec_9
Section sec_9.1
Section sec_9.2
Section sec_9.2.1
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
11 Section sec_9.2.2
Section sec_9.2.3
Section sec_9.2.4
Section sec_9.2.5
Section sec_9.2.6
Section sec_9.2.7
Section sec_9.2.8
Section sec_9.3
Section sec_9.3.1
Section sec_9.3.2
Section sec_10
Section sec_10.1
Section sec_10.2
Section sec_10.2.1
9.3 Counting of colonies
10 Expression of results
10.1 Method of calculation
10.2 Precision
12 Section sec_10.2.2
Section sec_10.2.3
Section sec_10.3
Section sec_10.3.1
Section sec_10.3.2
Section sec_10.3.3
10.3 Interpretation of test results
13 Section sec_11
11 Test report
14 Annex sec_A
Annex sec_A.1
Annex sec_A.2
Annex sec_A.3
AnnexĀ A
(informative)

Use of the critical difference for the interpretation of results

15 Reference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Reference ref_6
Reference ref_7
Bibliography

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BS EN ISO 4833-1:2013
$102.76