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BS EN ISO 19040-2:2022

BS EN ISO 19040-2:2022

$198.66

Water quality. Determination of the estrogenic potential of water and waste water – Yeast estrogen screen (A-YES, Arxula adeninivorans)

Published By Publication Date Number of Pages
BSI 2022 64
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PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
7 Foreword
9 1 Scope
10 2 Normative references
3 Terms and definitions
12 4 Principle
5 Interferences
6 Apparatus and materials
13 7 Reagents, media and test strains
17 8 Sampling and samples
8.1 General
8.2 Bottles and material for sampling
18 8.3 Bottles and material pre-cleaning
8.4 Sampling procedure
8.5 Transport of samples
8.6 Pretreatment of samples
19 8.7 Storage of samples
9 Procedure
9.1 Test set up
9.1.1 Preparation of the reference dilution series
9.1.2 Reactivation of the yeast
20 9.1.3 Negative control
9.1.4 Blank replicate
9.1.5 Sample dilution
21 9.1.6 Field blank
9.1.7 Plate set up
9.1.8 Inoculation of the test plate
22 9.2 Measurement
9.2.1 Measurement of the reporter gene activity
23 9.2.2 Measurement of the cell density
9.3 Calculations
9.3.1 Background correction
24 9.3.2 Calculation of the relative growth
25 9.3.3 Calculations for assessment of sample blanks
27 9.3.4 Calculation of the reporter gene induction
30 10 Validity criteria
31 11 Assessment criteria
12 Test report
13 Verification
33 Annex A (informative) Plate set up
34 Annex B (informative) Lyophilization of Arxula adeninivorans cells
36 Annex C (informative) Scheme of test principle
37 Annex D (informative) Test set up for chemicals and extracts
38 Annex E (informative) Preparation of dilution series
39 Annex F (informative) Performance data
49 Annex G (informative) Statistical assessment
50 Annex H (informative) Calculation of estradiol equivalents
53 Annex I (informative) Alternative test design for EEQ determination
54 Annex J (informative) Measurement of the lowest ineffective dilution (LID) of a waste water ā€” A simplified evaluation for testing of waste water
56 Annex K (informative) Example for statistical evaluation
63 Bibliography

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BS EN ISO 19040-2:2022
$198.66