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BS EN ISO 16212:2017

BS EN ISO 16212:2017

$142.49

Cosmetics. Microbiology. Enumeration of yeast and mould

Published By Publication Date Number of Pages
BSI 2017 30
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This document gives general guidelines for enumeration of yeast and mould present in cosmetics by counting the colonies on selective agar medium after aerobic incubation.

In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621 ) include those with low water activity or extreme pH values, hydro-alcoholic products, etc.

Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other methods (e.g. automated) can be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.

Yeast enumerated can be identified using suitable identification tests, for example, tests described in the standards listed in the Bibliography. Mould enumerated can be identified by other appropriate methods, if necessary.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principles
4.1 General
4.2 Plate count
4.3 Membrane filtration
11 5 Diluents, neutralizers and culture media
5.1 General
5.2 Neutralizing diluents and diluents
12 5.3 Diluent for yeast suspension (tryptone sodium chloride solution)
5.4 Culture media
13 6 Apparatus and glassware
7 Strain of microorganisms
14 8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension
9.2.1 General
9.2.2 Water-miscible products
9.2.3 Water-immiscible products
9.3 Counting methods
9.3.1 Dilutions for counting methods
15 9.3.2 Plate-count methods
10 Counting of colonies (plate counts and membrane filtration methods)
16 11 Expression of results
11.1 Method of calculation for plate count
17 11.2 Interpretation
18 12 Neutralization of the antifungicidal properties of the product
12.1 General
19 12.2 Preparation of inoculum
12.3 Suitability of counting methods
12.3.1 Principle
12.3.2 Suitability test of the pour-plate method
12.3.3 Suitability of the surface spread method
12.3.4 Suitability of the membrane filtration method
20 13 Test report
21 Annex A (informative) Other neutralizing diluents
23 Annex B (informative) Other diluents
24 Annex C (informative) Other culture media
26 Annex D (informative) Neutralizers of antifungicidal activity of preservatives and rinsing liquids
27 Bibliography

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BS EN ISO 16212:2017
$142.49