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BS EN ISO 15216-1:2017 – TC:2020 Edition

BS EN ISO 15216-1:2017 – TC:2020 Edition

$258.95

Tracked Changes. Microbiology of the food chain. Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR – Method for quantification

Published By Publication Date Number of Pages
BSI 2020 124
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PDF Catalog

PDF Pages PDF Title
71 Foreword
72 Introduction
73 1 Scope
2 Normative references
3 Terms and definitions
75 4 Principle
4.1 Virus extraction
76 4.2 RNA extraction
4.3 Real-time RT-PCR
4.4 Control materials
4.4.1 Process control virus
4.4.2 Double-stranded DNA (dsDNA) control
4.4.3 EC RNA control
77 4.5 Test results
5 Reagents
5.1 General
5.2 Reagents used as supplied
78 5.3 Prepared reagents
79 6 Equipment and consumables
81 7 Sampling
8 Procedure
8.1 General laboratory requirements
8.2 Virus extraction
8.2.1 Process control virus material
8.2.2 Negative process control
8.2.3 Food surfaces
8.2.4 Soft fruit, leaf, stem and bulb vegetables
82 8.2.5 Bottled water
83 8.2.6 Bivalve molluscan shellfish
8.3 RNA extraction
84 8.4 Real-time RT-PCR
8.4.1 General requirements
8.4.2 Real-time RT-PCR analysis
86 9 Interpretation of results
9.1 General
9.2 Construction of standard curves
87 9.3 Calculation of RT-PCR inhibition
9.4 Calculation of extraction efficiency
88 9.5 Sample quantification
89 10 Expression of results
11 Precision
11.1 Interlaboratory study
11.2 Repeatability
90 11.3 Reproducibility limit
12 Test report
91 Annex A (normative) Diagram of procedure
92 Annex B (normative) Composition and preparation of reagents and buffers
95 Annex C (informative) Real-time RT-PCR mastermixes and cycling parameters
96 Annex D (informative) Real-time RT-PCR primers and hydrolysis probes for the detection of HAV, norovirus GI and GII and mengo virus (process control)
99 Annex E (informative) Growth of mengo virus strain MC0 for use as a process control
100 Annex F (informative) RNA extraction using the NucliSENS® system
102 Annex G (informative) Generation of dsDNA control stocks
105 Annex H (informative) Generation of EC RNA stocks
107 Annex I (informative) Typical optical plate layout
109 Annex J (informative) Method validation studies and performance characteristics
120 Bibliography

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BS EN ISO 15216-1:2017 - TC
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