ASTM-E2526 2008

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E2526-08 Standard Test Method for Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells

Published By	Publication Date	Number of Pages
ASTM	2008	6

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Categories: 07.100.10 - Medical microbiology, ASTM

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Description

1.1 This test method provides a methodology to assess the cytotoxicity of suspensions of nanoparticulate materials in porcine proximal tubule cells (LLC-PK1) and human hepatocarcinoma cells (Hep G2) which represents potential target organs following systemic administration

1.2 This test method is part of the in vitro preclinical characterization cascade.

1.3 This test method consists of a protocol utilizing two methods for estimation of cytotoxicity, 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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PDF Pages	PDF Title
1	Scope Referenced Documents Terminology Summary of Test Method Significance and Use
2	Reagents and Materials Experimental Procedure
3	FIG. 1
4	Calculation or Interpretation of Results FIG. 2
5	FIG. 3
6	Rationale Precision and Bias Keywords

Additional information

Standard Title	E2526-08 Standard Test Method for Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells
Published Code	ASTM
Publication Date	2008
Pages Count	6
ICS Codes	07.100.10 - Medical microbiology

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ASTM-E2526 2008

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