ASTM-D6499 2024(Redline)

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D6499-24 Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products (Redline)

Published By	Publication Date	Number of Pages
ASTM	2024	14

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Category: ASTM

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Description

ASTM D6499-24

Redline Standard: Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products

ASTM D6499

Scope

1.1 This test method covers an immunological method to determine the amount of antigenic protein in Hevea Natural Rubber and its products using rabbit antisera specific for HNRL proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other product extracts which have been collected in order to measure the HNR levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.

1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

1.3 This test method can be used to determine the presence and amount of HNR protein contamination on test items.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Keywords

ELISA; Hevea natural rubber latex; proteins;

ICS Code

ICS Number Code 11.100 (Laboratory medicine)

DOI: 10.1520/D6499-24

Additional information

Standard Title	D6499-24 Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products (Redline)
Published Code	ASTM
Publication Date	2024
Pages Count	14