{"id":402589,"date":"2024-10-20T05:01:55","date_gmt":"2024-10-20T05:01:55","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-ts-177092022\/"},"modified":"2024-10-26T08:57:35","modified_gmt":"2024-10-26T08:57:35","slug":"bsi-pd-cen-ts-177092022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-ts-177092022\/","title":{"rendered":"BSI PD CEN\/TS 17709:2022"},"content":{"rendered":"

This document was developed to provide the methodology for the enumeration and determination of Azotobacter sp. in plant biostimulant products in accordance with the Regulation (EU) 2019\/1009 of the European Parliament and of the Council [1].<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
5<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
6<\/td>\nIntroduction <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions
4 Enumeration of Azotobacter spp.
4.1 General
4.2 Sample preparation
4.2.1 General
4.2.2 Liquid (based water) formulations
4.2.3 Liquid – based oil, emulsifiable concentrate (EC) formulations
4.2.4 Solid – Wettable Powder (WP) formulations <\/td>\n<\/tr>\n
8<\/td>\n4.2.5 Solid – Water dispersible granules (WDG) formulations
4.2.6 Solid \u2013 Pellets, granules, microgranules (slow release) formulations
4.2.7 Solid – substrate
4.3 Serial dilution <\/td>\n<\/tr>\n
9<\/td>\n4.4 Plate counts of Azotobacter sp in sterile diluent
4.5 Spread-plate counting with ASHBY SUCROSE AGAR [6] <\/td>\n<\/tr>\n
10<\/td>\n4.6 Calculation
5 Species determination of Azotobacter sp. via genetic analysis
5.1 General
5.2 Preparation of the sample for the genomic DNA extraction
5.2.1 Isolation and preparation of the microorganism
5.2.2 Sample concentration
5.2.3 DNA extraction and storage <\/td>\n<\/tr>\n
11<\/td>\n5.2.4 Partial PCR amplification of the 16S rRNA Genes <\/td>\n<\/tr>\n
13<\/td>\nAnnex A (informative) Formula of culture media <\/td>\n<\/tr>\n
15<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Plant biostimulants. Determination of Azotobacter spp.<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2022<\/td>\n16<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":402598,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-402589","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/402589","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/402598"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=402589"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=402589"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=402589"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}