{"id":381284,"date":"2024-10-20T03:10:51","date_gmt":"2024-10-20T03:10:51","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-134932021\/"},"modified":"2024-10-26T05:46:12","modified_gmt":"2024-10-26T05:46:12","slug":"bs-iso-134932021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-134932021\/","title":{"rendered":"BS ISO 13493:2021"},"content":{"rendered":"<p>This document specifies the liquid chromatographic (LC) method for the determination of chloramphenicol content of muscle tissue of meat, including livestock and poultry.<\/p>\n<p>This document specifies the liquid chromatography tandem mass spectrometry method (LC-MS\/MS) for the determination of chloramphenicol content of muscle tissue, casing, liver of meat and meat products, including livestock and poultry.<\/p>\n<p>This document specifies LC-MS\/MS as the reference method.<\/p>\n<p>The LC method is suitable for the determination of chloramphenicol content greater than 6,5&nbsp;mg\/kg.<\/p>\n<p>LC-MS\/MS is suitable for the determination of chloramphenicol content greater than 0,1&nbsp;?g\/kg.<\/p>\n<p>Test samples which have deteriorated cannot be analysed with this method.<\/p>\n<h4>PDF Catalog<\/h4>\n<table border=\"1px\" class=\"des-table\">\n<tr>\n<th>PDF Pages<\/th>\n<th>PDF Title<\/th>\n<\/tr>\n<tr>\n<td><b>2<b><\/td>\n<td>National foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>6<b><\/td>\n<td>Foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>7<b><\/td>\n<td>1 Scope <br \/> 2 Normative references <br \/> 3 Terms and definitions <br \/> 4 Principle <br \/> 4.1 Liquid chromatographic method  <\/td>\n<\/tr>\n<tr>\n<td><b>8<b><\/td>\n<td>4.2 Liquid chromatography tandem mass spectrometry method <br \/> 5 Sampling <br \/> 6 Preparation of test sample <br \/> 7 Test method of liquid chromatography <br \/> 7.1 Reagents <br \/> 7.1.2 Nitrogen, suitable for evaporating solvents. <br \/> 7.1.6 Acetonitrile, HPLC grade.  <\/td>\n<\/tr>\n<tr>\n<td><b>9<b><\/td>\n<td>7.2 Apparatus  <\/td>\n<\/tr>\n<tr>\n<td><b>10<b><\/td>\n<td>7.3 Procedure <br \/> 7.3.1 General <br \/> 7.3.2 Test portion <br \/> 7.3.3 Preparation of extract <br \/> 7.3.4 Solid-phase extraction <br \/> 7.3.5 Liquid-liquid extraction  <\/td>\n<\/tr>\n<tr>\n<td><b>11<b><\/td>\n<td>7.3.6 Chromatographic analysis <br \/> 7.4 Calculation  <\/td>\n<\/tr>\n<tr>\n<td><b>12<b><\/td>\n<td>7.5 Precision <br \/> 7.5.1 Interlaboratory test <br \/> 7.5.2 Repeatability <br \/> 7.5.3 Reproducibility  <\/td>\n<\/tr>\n<tr>\n<td><b>13<b><\/td>\n<td>8 Test method of liquid chromatography tandem mass spectrometry method (reference method) <br \/> 8.1 Reagents and materials <br \/> 8.1.8 Methanol and water solution I (volume ratio 2:8). <br \/> 8.1.9 Methanol and water solution II (volume ratio 1:1).  <\/td>\n<\/tr>\n<tr>\n<td><b>14<b><\/td>\n<td>8.1.17 Acetonitrile and water solution (volume ratio 1:9). <br \/> 8.2 Apparatus <br \/> 8.2.1 LC-MS\/MS equipment. <br \/> 8.3 Procedure <br \/> 8.3.1 General <br \/> 8.3.2 Test portion  <\/td>\n<\/tr>\n<tr>\n<td><b>15<b><\/td>\n<td>8.3.3 Preparation of extract <br \/> 8.3.4 Solid-phase extraction <br \/> 8.3.5 LC-MS\/MS analysis  <\/td>\n<\/tr>\n<tr>\n<td><b>17<b><\/td>\n<td>8.4 Calculation <br \/> 8.5 Precision <br \/> 8.5.1 Interlaboratory test <br \/> 8.5.2 Repeatability  <\/td>\n<\/tr>\n<tr>\n<td><b>18<b><\/td>\n<td>8.5.3 Reproducibility <br \/> 9 Test report  <\/td>\n<\/tr>\n<tr>\n<td><b>19<b><\/td>\n<td>Annex A (informative) HPLC chromatogram of chloramphenicol standard solution and LC-MS\/MS chromatogram of chloramphenicol standard solution  <\/td>\n<\/tr>\n<tr>\n<td><b>22<b><\/td>\n<td>Bibliography  <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p><b>Meat and meat products. Determination of chloramphenicol content. Reference method<\/b><\/p>\n<table class=\"shortdes-table\" style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/bsi\/\"><b>BSI<\/b><\/a><\/td>\n<td>2021<\/td>\n<td>24<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":381293,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[967,2641],"product_tag":[],"class_list":{"0":"post-381284","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-67-120-10","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/381284","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/381293"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=381284"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=381284"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=381284"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}