{"id":341390,"date":"2024-10-19T23:56:10","date_gmt":"2024-10-19T23:56:10","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-20976-12019\/"},"modified":"2024-10-25T23:08:15","modified_gmt":"2024-10-25T23:08:15","slug":"bs-en-iso-20976-12019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-20976-12019\/","title":{"rendered":"BS EN ISO 20976-1:2019"},"content":{"rendered":"

This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products.<\/p>\n

The use of this document can be extended to yeasts that do not form mycelium.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
4<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
7<\/td>\nForeword <\/td>\n<\/tr>\n
8<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
12<\/td>\n4 Principle
4.1 General <\/td>\n<\/tr>\n
14<\/td>\n4.2 Estimation of the growth potential <\/td>\n<\/tr>\n
15<\/td>\n4.3 Estimation of the growth kinetics parameters (lag time and maximum growth rate)
5 Apparatus
6 Culture media and reagents <\/td>\n<\/tr>\n
16<\/td>\n7 Study design and sampling
7.1 General
7.2 Setting decision criteria for growth potential
7.3 Number of batches and selection criteria
7.4 Preparation of the test units <\/td>\n<\/tr>\n
17<\/td>\n7.5 Number of test units to be inoculated
8 Selection of strains <\/td>\n<\/tr>\n
18<\/td>\n9 Preparation of the inoculum
9.1 General
9.2 Preparation of the vegetative cell suspensions
9.3 Preparation of the spore suspensions
10 Inoculation of the tests units <\/td>\n<\/tr>\n
19<\/td>\n11 Controls
11.1 Food controls
11.2 Control units <\/td>\n<\/tr>\n
20<\/td>\n12 Storage of the test units
12.1 General
12.2 Estimation of growth potential
12.3 Estimation of growth kinetics parameters (lag time and growth rate)
13 Analysis <\/td>\n<\/tr>\n
21<\/td>\n14 Expression of the results
14.1 General
14.2 Growth potential (\u0394) <\/td>\n<\/tr>\n
22<\/td>\n14.3 Growth kinetics parameters (lag time and growth rate)
15 Test report
15.1 General
15.2 Aim of the study and type of challenge test <\/td>\n<\/tr>\n
23<\/td>\n15.3 Experimental protocol
15.4 Sample analysis
15.5 Results <\/td>\n<\/tr>\n
24<\/td>\n15.6 Conclusions
15.7 Reference documents <\/td>\n<\/tr>\n
25<\/td>\nAnnex A (informative) Inter-batch variability assessment based on pH and aw <\/td>\n<\/tr>\n
26<\/td>\nAnnex B (normative) Minimum number of units to prepare for the challenge test study <\/td>\n<\/tr>\n
27<\/td>\nAnnex C (informative) Examples of protocols to prepare inocula <\/td>\n<\/tr>\n
30<\/td>\nAnnex D (informative) Examples of how to estimate growth potential, lag time and maximum growth rate from results of challenge tests <\/td>\n<\/tr>\n
34<\/td>\nAnnex E (informative) Use of simulation to assess a microbial population under different temperature conditions <\/td>\n<\/tr>\n
35<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of the food chain. Requirements and guidelines for conducting challenge tests of food and feed products – Challenge tests to study growth potential, lag time and maximum growth rate<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2019<\/td>\n38<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":341398,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-341390","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/341390","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/341398"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=341390"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=341390"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=341390"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}