This Technical Specification provides guidelines for the identification of the surface bound molecules using FT-IR of dehydrated gold nanoparticle (AuNPs) films both before and after nanomaterial (NM) cytotoxicity testing.<\/p>\n
\nNOTE 1 AuNPs may have surface bound ligands prior to testing and may be additionally covered (or coated) with organic- or bio-molecules during the cytotoxicity test.<\/p>\n<\/blockquote>\n
\nNOTE 2 Nucleic acids, amino acids, lipids or membrane components binding to AuNPs can be observed by FT-IR spectroscopy by detection of absorption bands corresponding to phosphodiester, amine or lipid, respectively, although the type of nucleic acids, proteins or lipid cannot be identified in detail based on IR spectra.<\/p>\n<\/blockquote>\n
PDF Catalog<\/h4>\n
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\n PDF Pages<\/th>\n PDF Title<\/th>\n<\/tr>\n \n 6<\/td>\n Foreword <\/td>\n<\/tr>\n \n 7<\/td>\n Introduction <\/td>\n<\/tr>\n \n 9<\/td>\n 1\tScope
2\tNormative references
3\tTerms and definitions <\/td>\n<\/tr>\n\n 11<\/td>\n 4\tSymbols and abbreviated terms
5\tSample preparation mode
5.1\tRemoval of unbound molecules <\/td>\n<\/tr>\n\n 14<\/td>\n 5.2\tDehydration
5.3\tScreening test for impurities in DW from sample tubes <\/td>\n<\/tr>\n\n 15<\/td>\n 6\tFT-IR measurement procedure
6.1\tGeneral
6.2\tATR method <\/td>\n<\/tr>\n\n 16<\/td>\n 6.3\tTransmission method <\/td>\n<\/tr>\n \n 17<\/td>\n 6.4\tDetermination of time required for complete purge <\/td>\n<\/tr>\n \n 18<\/td>\n 6.5\tLinear range of IR band intensity versus concentration <\/td>\n<\/tr>\n \n 19<\/td>\n 6.6\tLOD and LOQ determination <\/td>\n<\/tr>\n \n 20<\/td>\n 6.7\tRepeatability determination
7\tApplication examples
7.1\tDegrees of ligand exchange <\/td>\n<\/tr>\n\n 21<\/td>\n 7.2\tQualitative measurement of biomolecular binding <\/td>\n<\/tr>\n \n 23<\/td>\n Annex\u00a0A
\n(informative)<\/p>\nCase study for validation of ligand exchange <\/td>\n<\/tr>\n
\n 25<\/td>\n Annex\u00a0B
\n(informative)<\/p>\nCase study for qualitative measurement of biochemical moieties binding to the AuNP surface <\/td>\n<\/tr>\n
\n 28<\/td>\n Annex\u00a0C
\n(informative)<\/p>\nSelection guide for window materials <\/td>\n<\/tr>\n
\n 29<\/td>\n Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Surface characterization of gold nanoparticles for nanomaterial specific toxicity screening: FT-IR method<\/b><\/p>\n
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\n Published By<\/td>\n Publication Date<\/td>\n Number of Pages<\/td>\n<\/tr>\n \n BSI<\/b><\/a><\/td>\n 2013<\/td>\n 34<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":302215,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-302213","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/302213","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/302215"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=302213"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=302213"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=302213"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}