BSI PD ISO/TS 14101:2012:2013 Edition
$167.15
Surface characterization of gold nanoparticles for nanomaterial specific toxicity screening: FT-IR method
| Published By | Publication Date | Number of Pages |
| BSI | 2013 | 34 |
This Technical Specification provides guidelines for the identification of the surface bound molecules using FT-IR of dehydrated gold nanoparticle (AuNPs) films both before and after nanomaterial (NM) cytotoxicity testing.
NOTE 1 AuNPs may have surface bound ligands prior to testing and may be additionally covered (or coated) with organic- or bio-molecules during the cytotoxicity test.
NOTE 2 Nucleic acids, amino acids, lipids or membrane components binding to AuNPs can be observed by FT-IR spectroscopy by detection of absorption bands corresponding to phosphodiester, amine or lipid, respectively, although the type of nucleic acids, proteins or lipid cannot be identified in detail based on IR spectra.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 11 | 4 Symbols and abbreviated terms 5 Sample preparation mode 5.1 Removal of unbound molecules |
| 14 | 5.2 Dehydration 5.3 Screening test for impurities in DW from sample tubes |
| 15 | 6 FT-IR measurement procedure 6.1 General 6.2 ATR method |
| 16 | 6.3 Transmission method |
| 17 | 6.4 Determination of time required for complete purge |
| 18 | 6.5 Linear range of IR band intensity versus concentration |
| 19 | 6.6 LOD and LOQ determination |
| 20 | 6.7 Repeatability determination 7 Application examples 7.1 Degrees of ligand exchange |
| 21 | 7.2 Qualitative measurement of biomolecular binding |
| 23 | Annex A (informative) Case study for validation of ligand exchange |
| 25 | Annex B (informative) Case study for qualitative measurement of biochemical moieties binding to the AuNP surface |
| 28 | Annex C (informative) Selection guide for window materials |
| 29 | Bibliography |
