{"id":410674,"date":"2024-10-20T05:41:30","date_gmt":"2024-10-20T05:41:30","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-19040-32022\/"},"modified":"2024-10-26T10:28:58","modified_gmt":"2024-10-26T10:28:58","slug":"bs-en-iso-19040-32022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-19040-32022\/","title":{"rendered":"BS EN ISO 19040-3:2022"},"content":{"rendered":"

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
4<\/td>\nEuropean foreword
Endorsement notice <\/td>\n<\/tr>\n
7<\/td>\nForeword <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
12<\/td>\n4 Interferences
5 Principle
6 Apparatus and materials <\/td>\n<\/tr>\n
13<\/td>\n7 Reagents, cells and media <\/td>\n<\/tr>\n
17<\/td>\n8 Sampling and samples
8.1 General
8.2 Bottles and material for sampling
8.3 Bottles and material pre-cleaning
8.4 Sampling procedure
8.5 Transport of samples <\/td>\n<\/tr>\n
18<\/td>\n8.6 Pretreatment of sample
8.7 Storage of samples
9 Procedure
9.1 Cell culture maintenance
9.1.1 Freezing cells
9.1.2 Starting a new cell culture <\/td>\n<\/tr>\n
19<\/td>\n9.1.3 Culturing cells
9.2 Human cell reporter gene assay test procedure
9.2.1 Seeding the cells (day 1)
9.2.2 Preparation of the E2-reference (day 2) <\/td>\n<\/tr>\n
20<\/td>\n9.2.3 Preparation of the sample dilutions (day 2)
9.2.4 Field blank
9.2.5 Exposing the cells (day 2) <\/td>\n<\/tr>\n
21<\/td>\n9.2.6 Harvesting the cells (day 3)
9.2.7 Measurement of luminescence (day 3)
9.3 Data analysis
9.3.1 Calculation of the reporter gene induction <\/td>\n<\/tr>\n
22<\/td>\n9.3.2 Calculation of the percentage of maximum response
9.3.3 Calculation of the dose-response curve
10 Validity criteria
10.1 Validity criteria for the assay <\/td>\n<\/tr>\n
23<\/td>\n10.2 Validity criteria for samples
11 Assessment criteria
12 Test report <\/td>\n<\/tr>\n
24<\/td>\nAnnex A (informative) Settings of the luminometer <\/td>\n<\/tr>\n
25<\/td>\nAnnex B (informative) Plate setup <\/td>\n<\/tr>\n
26<\/td>\nAnnex C (informative) Bioassay characteristics and details <\/td>\n<\/tr>\n
28<\/td>\nAnnex D (informative) Test set up for chemicals and extracts <\/td>\n<\/tr>\n
30<\/td>\nAnnex E (informative) Preparation of dilution series <\/td>\n<\/tr>\n
31<\/td>\nAnnex F (informative) Performance data <\/td>\n<\/tr>\n
41<\/td>\nAnnex G (informative) Statistical assessment <\/td>\n<\/tr>\n
42<\/td>\nAnnex H (informative) Calculation of 17\u03b2-estradiol equivalents <\/td>\n<\/tr>\n
44<\/td>\nAnnex I (informative) Measurement of the lowest ineffective dilution (LID) of waste water \u2014 A simplified evaluation for testing of waste water <\/td>\n<\/tr>\n
47<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Water quality. Determination of the estrogenic potential of water and waste water – In vitro human cell-based reporter gene assay<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2022<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":410684,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[218,2641],"product_tag":[],"class_list":{"0":"post-410674","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-13-060-70","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/410674","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/410684"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=410674"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=410674"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=410674"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}