{"id":402162,"date":"2024-10-20T04:59:52","date_gmt":"2024-10-20T04:59:52","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-22942-12022\/"},"modified":"2024-10-26T08:52:13","modified_gmt":"2024-10-26T08:52:13","slug":"bs-iso-22942-12022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-22942-12022\/","title":{"rendered":"BS ISO 22942-1:2022"},"content":{"rendered":"

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
7<\/td>\nForeword <\/td>\n<\/tr>\n
8<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
11<\/td>\n4 Principle
5 Development of an isoPCR method
5.1 General
5.2 Intended purpose
5.3 Scientific basis <\/td>\n<\/tr>\n
14<\/td>\n5.4 Units of measurement
5.5 Method validation
5.6 Performance criteria
5.6.1 General
5.6.2 Sensitivity <\/td>\n<\/tr>\n
15<\/td>\n5.6.3 Nucleic acid extract quality
5.6.4 Applicability
5.6.5 Nucleic acid sequence specificity
5.6.6 Precision <\/td>\n<\/tr>\n
16<\/td>\n5.6.7 Accuracy
5.6.8 Selectivity
5.6.9 Linearity
5.6.10 Limit of detection (LOD) <\/td>\n<\/tr>\n
17<\/td>\n5.6.11 Limit of quantification (LOQ) <\/td>\n<\/tr>\n
18<\/td>\n5.6.12 Range
5.6.13 Robustness <\/td>\n<\/tr>\n
19<\/td>\n6 General laboratory and procedural requirements
6.1 Competence
6.2 Sample preparation
6.2.1 General
6.2.2 Obtaining a representative sample
6.2.3 Preparation of the test portion <\/td>\n<\/tr>\n
20<\/td>\n6.2.4 Nucleic acid extraction
6.3 Use of controls
6.3.1 General
6.3.2 Environmental controls
6.3.3 Positive controls
6.3.4 Negative controls
6.3.5 Extraction controls <\/td>\n<\/tr>\n
21<\/td>\n6.4 Workspace organization
6.4.1 General
6.4.2 Design of the workspace \u2014 Laboratory design
6.4.3 Design of non-laboratory workspaces
6.4.4 Personnel <\/td>\n<\/tr>\n
22<\/td>\n6.4.5 Apparatus and equipment
7 Materials and reagents
8 Interpretation of results
8.1 General
8.2 Interpretation of controls <\/td>\n<\/tr>\n
23<\/td>\n8.3 Expression of results
8.3.1 General
8.3.2 Expression of a negative result <\/td>\n<\/tr>\n
24<\/td>\n8.3.3 Expression of a positive result
8.3.4 Expression of quantitative results
8.3.5 Expression of ambiguous results
9 Test report <\/td>\n<\/tr>\n
26<\/td>\nAnnex A (informative) Minimum information for an isoPCR experiment (MIIPCRE) <\/td>\n<\/tr>\n
29<\/td>\nAnnex B (normative) Use of controls <\/td>\n<\/tr>\n
30<\/td>\nAnnex C (informative) Examples of isothermal nucleic acid isoPCR amplification results <\/td>\n<\/tr>\n
31<\/td>\nAnnex D (informative) Loop mediated isothermal amplification (LAMP) <\/td>\n<\/tr>\n
34<\/td>\nAnnex E (informative) Rolling circle amplification (RCA) <\/td>\n<\/tr>\n
35<\/td>\nAnnex F (informative) Helicase dependent amplification (HDA) <\/td>\n<\/tr>\n
37<\/td>\nAnnex G (informative) Recombinase polymerase amplification (RPA) <\/td>\n<\/tr>\n
39<\/td>\nAnnex H (informative) Strand displacement amplification (SDA) <\/td>\n<\/tr>\n
41<\/td>\nAnnex I (informative) Nucleic acid sequence based amplification (NASBA) <\/td>\n<\/tr>\n
44<\/td>\nAnnex J (informative) Cas9nAR amplification <\/td>\n<\/tr>\n
46<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Molecular biomarker analysis. Isothermal polymerase chain reaction (isoPCR) methods – General requirements<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2022<\/td>\n50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":402171,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-402162","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/402162","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/402171"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=402162"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=402162"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=402162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}