{"id":338366,"date":"2024-10-19T23:40:34","date_gmt":"2024-10-19T23:40:34","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-16-30337114-dc\/"},"modified":"2024-10-25T22:46:42","modified_gmt":"2024-10-25T22:46:42","slug":"bsi-16-30337114-dc","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-16-30337114-dc\/","title":{"rendered":"BSI 16\/30337114 DC"},"content":{"rendered":"
PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
---|---|---|---|---|---|---|---|
4<\/td>\n | Contents Page <\/td>\n<\/tr>\n | ||||||
5<\/td>\n | European foreword <\/td>\n<\/tr>\n | ||||||
6<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | 1 Scope 2 Normative references 3 Principle 4 Reagents <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 5 Apparatus <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 6 Procedure 6.1 General 6.2 Preparation of the sample extract 6.3 Clean-up of the extracts 6.3.1 Carrez precipitation 6.3.2 Solid phase extraction <\/td>\n<\/tr>\n | ||||||
11<\/td>\n | 6.4 HPLC-MS\/MS measurement 6.4.1 High performance liquid chromatography (HPLC) 6.4.2 Identification and quantification by mass spectrometry (HPLC-MS\/MS) Table 1 \u2014 Mass spectrometric transitions used for the identification and quantification of acrylamide <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 6.5 Measurement with GC-MS 6.5.1 Derivatisation and sample preparation for gas chromatography 6.5.2 Gas chromatography 6.5.3 Identification and quantification by mass spectrometry <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | Table 2 \u2014 Observed ions 7 Calibration 7.1 General advice 7.2 Determination of linearity and definition of the working range 7.3 Calibration with internal standard 7.4 Determination of the laboratory specific recovery <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 8 Evaluation 8.1 Criteria for identification 8.2 Calculation and final results 9 Precision data 9.1 General 9.2 Repeatability <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | 9.3 Reproducibility Table 3 \u2014 Precision data for the determination of acrylamide in coffee 9.4 Recovery 10 Measurement uncertainty 11 Test report <\/td>\n<\/tr>\n | ||||||
16<\/td>\n | Annex A (informative) Performance characteristics Table A.1 \u2014 Inter-laboratory study results (for both HPLC-and GC variants)0F ) <\/td>\n<\/tr>\n | ||||||
17<\/td>\n | Annex B (informative) Examples of absorber materials Table B.1 \u2014 Examples of suitable sorbents for the SPE clean-up (HPLC and GC, see 6.3) Table B.2 \u2014 Examples of suitable sorbents for the SPE clean-up of the GC extract (see 6.5.1) <\/td>\n<\/tr>\n | ||||||
18<\/td>\n | Annex C (informative) Examples for recommended columns and analysis conditions C.1 Generally suitable chromatographic columns Table C.1 \u2014 Analytical conditions for selected HPLC columns Table C.2 \u2014 GC capillary columns <\/td>\n<\/tr>\n | ||||||
19<\/td>\n | C.2 Example method and approximate retention time for HPLC-MS\/MS C.2.1 Example A Table C.3 \u2014 Chromatographic conditions for example A C.2.2 Example B Table C.4 \u2014 Chromatographic conditions for example B C.3 Example of suitable conditions and approximate retention time for GC-MS analysis <\/td>\n<\/tr>\n | ||||||
20<\/td>\n | Table C.5 \u2014 GC method: Example of suitable conditions and approximate retention time for GC-MS <\/td>\n<\/tr>\n | ||||||
21<\/td>\n | C.4 Example chromatograms for HPLC-MS\/MS Figure C.1 \u2014 D3-acrylamide <\/td>\n<\/tr>\n | ||||||
22<\/td>\n | Figure C.2 \u2014 Acrylamide, native <\/td>\n<\/tr>\n | ||||||
23<\/td>\n | C.5 Example of chromatogram for GC-MS Figure C.3 \u2014 Total ion chromatogram (scan mode) <\/td>\n<\/tr>\n | ||||||
24<\/td>\n | Figure C.4 \u2014 Extracted ion, mass 149 (quantifier ion) Figure C.5 \u2014 Extracted ion, mass 106 (qualifier ion) <\/td>\n<\/tr>\n | ||||||
25<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" BS EN 16987. Foodstuffs. Determination of acrylamide in coffee and coffee products by HPLC-MS\/MS and GC-MS<\/b><\/p>\n |